Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos
نویسندگان
چکیده
منابع مشابه
Quantitative RT-PCR amplification of RNA in single mouse oocytes and preimplantation embryos.
We describe a simple whole-cell method for quantitative reverse transcription (RT) PCR amplification of RNA that consistently allows the analysis of trace amounts of RNA, such as those carried by a fraction of a single mouse oocyte or preimplantation embryo, without organic extraction. The method is based on a preliminary genomic DNA digestion by DNase I in the presence of Mn++ and a subsequent...
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ژورنال
عنوان ژورنال: BMC Developmental Biology
سال: 2007
ISSN: 1471-213X
DOI: 10.1186/1471-213x-7-58